Random and direct mutagenesis to enhance protein secretion inAshbya gossypii
نویسندگان
چکیده
منابع مشابه
Random and direct mutagenesis to enhance protein secretion in Ashbya gossypii
To improve the general secretion ability of the biotechnologically relevant fungus Ashbya gossypii, random mutagenesis with ethyl methane sulfonate (EMS) was performed. The selection and screening strategy followed revealed mutants with improved secretion of heterologous Trichoderma reesei endoglucanase I (EGI), native α-amylase and/or native β-glucosidase. One mutant, S436, presented 1.4- to 2...
متن کاملLocalization of a protein-DNA interface by random mutagenesis.
The type I restriction and modification enzymes do not possess obvious DNA-binding motifs within their target recognition domains (TRDs) of 150-180 amino acids. To identify residues involved in DNA recognition, changes were made in the amino-TRD of EcoKI by random mutagenesis. Most of the 101 substitutions affecting 79 residues had no effect on the phenotype. Changes at only seven positions cau...
متن کاملTranscription-Associated Mutagenesis Increases Protein Sequence Diversity More Effectively than Does Random Mutagenesis in Escherichia coli
BACKGROUND During transcription, the nontranscribed DNA strand becomes single-stranded DNA (ssDNA), which can form secondary structures. Unpaired bases in the ssDNA are less protected from mutagens and hence experience more mutations than do paired bases. These mutations are called transcription-associated mutations. Transcription-associated mutagenesis is increased under stress and depends on ...
متن کاملInfluence of lipoteichoic acid D-alanylation on protein secretion in Lactococcus lactis as revealed by random mutagenesis.
Lactococcus lactis, a food-grade nonpathogenic lactic acid bacterium, is a good candidate for the production of heterologous proteins of therapeutic interest. We examined host factors that affect secretion of heterologous proteins in L. lactis. Random insertional mutagenesis was performed with L. lactis strain MG1363 carrying a staphylococcal nuclease (Nuc) reporter cassette in its chromosome. ...
متن کاملDoes Foreign Direct Investment Enhance Economic Growth: Insight from Bangladesh
This study deals with foreign direct investment and its impact on economic growth in the context of Bangladesh. The main objective of our study is to evaluate the impact of FDI on the economy of Bangladesh based on the secondary data covering year 2013-2017. This study considers FDI as a dependent variable and GDP, export and investment as independent variables and is used as the indicators of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Bioengineered
سال: 2013
ISSN: 2165-5979,2165-5987
DOI: 10.4161/bioe.24653